Free real time pcr primer design software

Access information specific to your industry's workflow. Pivot as your targets change with scalable chemistries and automated workflows. Resources New to RealTimeDesign software? Start Design. Invalid email address or password. Quick order. View products. Ask A Scientist.

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Ask A Question. Who would you like to reach? An intermediate selection of primer parameters are also there to tweak. All that is required is a target sequence, which is copied and pasted into the program, then you have the option to tweak the design parameters.

The output also includes a suggested annealing temperature to use for each primer pair. Do you have any free PCR primer design software suggestions to add to the list? Leave a comment below with your preferred choice and I will be sure to update the list.

Hi Thank You Steven for the great help. I need to know if any of these softwares can help me design multiplex pcr primer sets I need something to help me analyse Self Complementary structures.

Hi Nima Many thanks for your comment. For multiplex primer design tools that are free, your choices are rather limited. I did see a recent one called oli2go. I have never used this, but it does look like it may help you out. If you find any more, please let me know and I can update the list.

Best wishes, Steven. Save my name, email, and website in this browser for the next time I comment. Sign in. Forgot your password?

Get help. Privacy Policy. Password recovery. Top Tip Bio. Thank you so much Dr Steven. I need to know if any of these softwares can help me design multiplex pcr primer sets I need something to help me analyse Self Complementary structures Regards.

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